The fixture was originally scheduled to take place last Saturday, but it was cancelled as Wanderers players boycotted over unpaid wages.
Bolton have been told they must fulfil their two outstanding fixtures – the Brentford clash as well as the final day trip to Nottingham Forest on Sunday – with the EFL to decide on Thursday when the Brentford match will take place.
The Brentford game will not be rearranged for this week, with the EFL confirming it could take place after the last round of fixtures has been completed.
“The EFL has this morning confirmed that Bolton Wanderers’ suspended Sky Bet Championship fixture with Brentford will not be played this week,” a statement released by the governing body read.
“The EFL Board will now determine on Thursday 2 May 2019 as to what arrangements are to be put in place in regards to the fixture and Bolton Wanderers will be instructed accordingly.