The 30-year-old missed the FA Cup third round draw at Middlesbrough at the weekend, with the Premier League confirming on Tuesday that he has since gone under the knife.
The latest news is a big blow for Spurs boss Jose Mourinho, who is already without a number of key players having lost talisman Harry Kane to a hamstring tear.
Kane suffered his injury during the 1-0 Premier League defeat at Southampton on New Year’s Day, with Sissoko’s knee issue also occurring at St Mary’s.
“Following a clinical assessment and scans, Moussa Sissoko has today (Tuesday) undergone surgery to the medial collateral ligament in his right knee,” a Tottenham statement read.
“The France international sustained the injury in our fixture against Southampton, resulting in him missing our recent FA Cup tie at Middlesbrough.
“Our medical staff will monitor Moussa’s recovery before he commences rehabilitation with a view to returning to training in early April.”