Harlequins full-back Mike Brown has been left out of the 25-man England squad retained ahead of this Saturday’s international against South Africa.
Chris Ashton, Elliot Daly, Jonny May and Jack Nowell are preferred as the back three options, with Daly likely to continue at full-back.
In addition Courtney Lawes (Northampton Saints) will remain in Portugal with Eddie Jones’ squad as he continues his rehabilitation.
Lawes rejoined the squad in Portugal on Monday night, having returned to England for treatment on a back injury at the weekend.
Forwards coach Steve Borthwick said they were hopeful to have Lawes available for next weekend’s fixture against New Zealand.
The group also includes uncapped forward trio Zach Mercer, Ben Moon and Elliott Stooke, but there is no place for Ben Morgan.
The 23-man matchday squad will be announced on Thursday before returning to London.
Forwards: Tom Curry (Sale Sharks), Charlie Ewels (Bath Rugby), Jamie George (Saracens), Dylan Hartley (Northampton Saints) co-captain, Alec Hepburn (Exeter Chiefs), Maro Itoje (Saracens), George Kruis (Saracens), Zach Mercer (Bath Rugby), Ben Moon (Exeter Chiefs), Brad Shields (Wasps), Kyle Sinckler (Harlequins), Elliott Stooke (Bath Rugby), Harry Williams (Exeter Chiefs), Mark Wilson (Newcastle Falcons)
Backs: Chris Ashton (Sale Sharks), Danny Care (Harlequins), Elliot Daly (Wasps), Owen Farrell (Saracens) co-captain, George Ford (Leicester Tigers), Jonny May (Leicester Tigers), Jack Nowell (Exeter Chiefs), Henry Slade (Exeter Chiefs), Ben Te’o (Worcester Warriors), Manu Tuilagi (Leicester Tigers), Ben Youngs (Leicester Tigers)