Barca boss Ernesto Valverde appears to be resting Messi, who scored his 53rd career hat-trick in a 5-2 win over Mallorca on Saturday.
Valverde can afford to be without his star man for the Inter clash with his side already through to the knockout stages.
Inter Milan and Borussia Dortmund are fighting it out to join them in the last-32.
Both clubs are level on seven points, however, Antonio Conte’s men occupy second spot due to their better head-to-head record against the German giants.
Messi scored in a 3-1 win over Dortmund on matchday five, which saw Inter leapfrog them into second with their victory in Prague.
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Dortmund are likely to beat Slavia Prague, who are winless in their five group games, while Inter could fancy their chances against a potentially weakened Barcelona team.