Marvin Orie will captain the Golden Lions in the absence of Ross Cronje when they take on the Blue Bulls at Loftus Versfeld on Saturday.
Cronje, who captained the side last weekend, has been left out of the squad altogether because he will tour Australasia with the Springboks next week for the away leg of the Rugby Championship.
The Lions do, however, welcome back centre Lionel Mapoe, who scored his maiden Springbok try last weekend against Argentina, while there are other Boks in the form of Andries Coetzee, Courtnall Skosan and Orie in the side.
Golden Lions: 15 Andries Coetzee, 14 Sylvian Mahuza, 13 Lionel Mapoe, 12 Howard Mnisi, 11 Courtnall Skosan, 10 Shaun Reynolds, 9 Dillon Smit, 8 Hacjivah Dayimani, 7 Len Massyn, 6 James Venter, 5 Marvin Orie (c), 4 Rhyno Herbst, 3 Johannes Jonker, 2 Corne Fourie, 1 Sti Sithole
Replacements: 16 Pieter Jansen, 17 Danie Mienie, 18 Jacobie Adriaanse, 19 Reinhard Nothnagel, 20 Vincent Tshituka, 21 Madosh Tambwe, 22 Wandisile Simelane
Date: Saturday, September 1
Venue: Loftus Versfeld
Kick-off: 16:05 local (15:05 BST, 14:05 GMT)
Referee: Rasta Rasivhenge
Assistant referees: Jaco Pretorius, Ricus van der Hoven
Television match official: Willie Vos